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bebm bronchial epithelial cell growth basal medium  (Lonza)


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    Lonza bebm bronchial epithelial cell growth basal medium
    Bebm Bronchial Epithelial Cell Growth Basal Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bronchial+epithelial+basal+medium+(bebm)/bronchial+epithelial+growth+medium/pmc12118631-236-5-12
    Average 90 stars, based on 1 article reviews
    bebm bronchial epithelial cell growth basal medium - by Bioz Stars, 2026-10
    90/100 stars

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    Modification:

    Article Title: Bronchial epithelial cells release inflammatory markers linked to airway inflammation and remodeling in response to TLR5 ligand flagellin
    Article Snippet: .. NHBE cells were fed a 1:1 mixture of bronchial epithelial basal medium (BEBM, Lonza, USA) and Dulbecco Modified Eagle's Medium (DMEM, Corning, USA), and differentiated in ALI culture for 14–16 days. .. The basal cell surface of an NHBE cell-ALI culture was treated with purified flagellin from Pseudomonas aeruginosa (InvivoGen, USA) at 10 and 100 ng/ml for 3 and 24 h. In the experiments with transforming growth factor beta (TGF-β), 10 ng/ml of TGF-β1 or TGF-β2 (R&D Systems, South Korea) were pretreated 1 h before 100 ng/ml flagellin treatment.

    Article Title: Cannabinoid WIN55,212-2 restores bronchial epithelium by regulating oxidative stress and STAT6 phosphorylation.
    Article Snippet: .. Airway epithelial cell cultures required several media depending on the differentiation stage: bronchial epithelial basal medium (BEBM, Lonza), Dulbecco ́s modified Eagle medium (DMEM, Fisher), Opti-MEM (Gibco), as well as bronchial epithelial growth medium (BEGM) SingleQuots (Lonza) and all-trans-retinoic acid (ATRA, SigmaAldrich) supplementations were used. .. Additionally, cultures of human bronchial epithelial spheroid (bronchospheroids) included the use of growth factor reduced extracellular matrix gel (GFR-ECM, Sigma).

    Article Title: Intratympanic administration of dexamethasone attenuates radiation induced damage to middle ear mucosa
    Article Snippet: All histological analyses were performed via a light microscope (Olympus BX51; Olympus, Tokyo, Japan) at 40x magnification. .. Human middle ear epithelial (HMEE) cells were maintained in a mixture of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA, USA) and bronchial epithelial basal medium (BEBM, Lonza, Walkersville, MD, USA) at a 1:1 ratio . ..

    Article Title: Bronchial epithelial cells release inflammatory markers linked to airway inflammation and remodeling in response to TLR5 ligand flagellin.
    Article Snippet: Primary bronchial epithelial cell cultures NHBE cells (Lonza, USA) at passages 2–3 were plated on 12-well transwell plates (Corning, USA) coated with type 1 collagen (Corning, USA) as previously described.20 When confluent (5–7 days), cells were exposed to air by removing media from the apical surfaces of cells. .. NHBE cells were fed a 1:1 mixture of bronchial epithelial basal medium (BEBM, Lonza, USA) and Dulbecco Modified Eagle’s Medium (DMEM, Corning, USA), and differentiated in ALI culture for 14–16 days. .. The basal cell surface of an NHBE cell-ALI culture was treated with purified flagellin from Pseudomonas aeruginosa (InvivoGen, USA) at 10 and Volume 16, No. 6, June 2023 3 100 ng/ml for 3 and 24 h. In the experiments with transforming growth factor beta (TGF-b), 10 ng/ml of TGF-b1 or TGF-b2 (R&D Systems, South Korea) were pretreated 1 h before 100 ng/ml flagellin treatment.

    Cell Culture:

    Article Title: Therapeutic potential of 18-β-glycyrrhetinic acid-loaded poly (lactic-co-glycolic acid) nanoparticles on cigarette smoke-induced in-vitro model of COPD.
    Article Snippet: .. Human airway-derived epithelial cells (BCi-NS1.1) from Weill Cornell were cultured in bronchial epithelial basal medium (BEBM, Lonza, USA) supplemented with specific growth factors [44]. .. MTT (3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide), dimethyl sulphoxide (DMSO), dichlorodihydrofluorescein diacetate (DCF-DA), were obtained from Sigma-Aldrich, St. Louis, MO.

    Article Title: Downregulation of angulin-1/LSR induces malignancy via upregulation of EGF-dependent claudin-2 and TGF-β-dependent cell metabolism in human lung adenocarcinoma A549 cells.
    Article Snippet: .. After centrifugation at 1000g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville MD, USA) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM® SingleQuots® (Lonza Walkersville MD, USA, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/ml penicillin, 100 mg/ml streptomycin and 50 μg/ml amphotericin-B on 35- and 60-mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35-μm glass-wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/ml of 0.1% acetic acid). ..

    Article Title: The Roles and Regulatory Mechanisms of Tight Junction Protein Cingulin and Transcription Factor Forkhead Box Protein O1 in Human Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial Cells
    Article Snippet: .. After centrifugation at 1000× g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM ® SingleQuots ® (Lonza Walkersville, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/mL penicillin, 100 μg/mL streptomycin, and 50 μg/mL amphotericin-B on 35 and 60 mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35 mm glass wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/mL of 0.1% acetic acid). ..

    Article Title: Promotion of epithelial repair
    Article Snippet: .. Cell Culture and Reagents Bronchial epithelial basal medium (BEBMTM) and bronchial epithelial cell growth medium (BEGMTM) were purchased from LONZA (Basel, Switzerland). ..

    Centrifugation:

    Article Title: Downregulation of angulin-1/LSR induces malignancy via upregulation of EGF-dependent claudin-2 and TGF-β-dependent cell metabolism in human lung adenocarcinoma A549 cells.
    Article Snippet: .. After centrifugation at 1000g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville MD, USA) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM® SingleQuots® (Lonza Walkersville MD, USA, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/ml penicillin, 100 mg/ml streptomycin and 50 μg/ml amphotericin-B on 35- and 60-mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35-μm glass-wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/ml of 0.1% acetic acid). ..

    Article Title: The Roles and Regulatory Mechanisms of Tight Junction Protein Cingulin and Transcription Factor Forkhead Box Protein O1 in Human Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial Cells
    Article Snippet: .. After centrifugation at 1000× g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM ® SingleQuots ® (Lonza Walkersville, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/mL penicillin, 100 μg/mL streptomycin, and 50 μg/mL amphotericin-B on 35 and 60 mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35 mm glass wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/mL of 0.1% acetic acid). ..

    Isolation:

    Article Title: Downregulation of angulin-1/LSR induces malignancy via upregulation of EGF-dependent claudin-2 and TGF-β-dependent cell metabolism in human lung adenocarcinoma A549 cells.
    Article Snippet: .. After centrifugation at 1000g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville MD, USA) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM® SingleQuots® (Lonza Walkersville MD, USA, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/ml penicillin, 100 mg/ml streptomycin and 50 μg/ml amphotericin-B on 35- and 60-mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35-μm glass-wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/ml of 0.1% acetic acid). ..

    Article Title: The Roles and Regulatory Mechanisms of Tight Junction Protein Cingulin and Transcription Factor Forkhead Box Protein O1 in Human Lung Adenocarcinoma A549 Cells and Normal Lung Epithelial Cells
    Article Snippet: .. After centrifugation at 1000× g for 2 min, isolated cells were cultured in bronchial epithelial basal medium (BEBM, Lonza Walkersville) containing 4% fetal bovine serum (FBS) (CCB, Nichirei Bioscience, Tokyo, Japan) and supplemented with BEGM ® SingleQuots ® (Lonza Walkersville, including 0.4% bovine pituitary extract, 0.1% insulin, 0.1% hydrocortisone, 0.1% gentamicin, amphotericin-B [GA-1000], 0.1% retinoic acid, 0.1% transferrin, 0.1% triiodothyronine, 0.1% epinephrine, 0.1% human epidermal growth factor), 100 U/mL penicillin, 100 μg/mL streptomycin, and 50 μg/mL amphotericin-B on 35 and 60 mm culture dishes (Corning Glass Works, Corning, NY, USA) or in 35 mm glass wells (Iwaki, Chiba, Japan), coated with rat tail collagen (500 μg of dried tendon/mL of 0.1% acetic acid). ..



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    Spearman correlation analysis between E-cadherin percent expression and <t>epithelial</t> macrophages per mm of the reticular basement membrane (RBM) from samples of ACO (A) , N-cadherin percent expression and epithelial macrophages per mm of RBM from samples of ACO (B) , S100A4 positive basal cells and epithelial macrophages per mm of RBM from samples of ACO (C) , and vimentin positive basal cells and epithelial macrophages per mm of RBM (D) from samples of ACO.
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    bronchial epithelial cells basal medium (bebm)  (Lonza)
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    Spearman correlation analysis between E-cadherin percent expression and <t>epithelial</t> macrophages per mm of the reticular basement membrane (RBM) from samples of ACO (A) , N-cadherin percent expression and epithelial macrophages per mm of RBM from samples of ACO (B) , S100A4 positive basal cells and epithelial macrophages per mm of RBM from samples of ACO (C) , and vimentin positive basal cells and epithelial macrophages per mm of RBM (D) from samples of ACO.
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    Image Search Results


    Spearman correlation analysis between E-cadherin percent expression and epithelial macrophages per mm of the reticular basement membrane (RBM) from samples of ACO (A) , N-cadherin percent expression and epithelial macrophages per mm of RBM from samples of ACO (B) , S100A4 positive basal cells and epithelial macrophages per mm of RBM from samples of ACO (C) , and vimentin positive basal cells and epithelial macrophages per mm of RBM (D) from samples of ACO.

    Journal: Frontiers in Immunology

    Article Title: Epithelial-to-mesenchymal transition is an active process in the large airways of patients with asthma-COPD overlap and partially abrogated by inhaled corticosteroid treatment: a bronchoscopy endobronchial biopsy study

    doi: 10.3389/fimmu.2025.1531279

    Figure Lengend Snippet: Spearman correlation analysis between E-cadherin percent expression and epithelial macrophages per mm of the reticular basement membrane (RBM) from samples of ACO (A) , N-cadherin percent expression and epithelial macrophages per mm of RBM from samples of ACO (B) , S100A4 positive basal cells and epithelial macrophages per mm of RBM from samples of ACO (C) , and vimentin positive basal cells and epithelial macrophages per mm of RBM (D) from samples of ACO.

    Article Snippet: Cigarette smoke extract (CSE) was prepared by bubbling smoke from one filter-less Kentucky research cigarette, 3R4F, which contains 9.5 mg of tar and 0.8 mg of nicotine, through 10 mL of Bronchial Epithelial Basal Medium (BEBM, Lonza) at a rate of one cigarette every 5 minutes ( ).

    Techniques: Expressing, Membrane

    Relative expression changes of N-cadherin and E-cadherin in human-derived lung primary epithelial cells (HC and ACO patients) post induction with Th-2 cytokine IL-13, CSE and EMT activator TGF-β. # p<0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: Frontiers in Immunology

    Article Title: Epithelial-to-mesenchymal transition is an active process in the large airways of patients with asthma-COPD overlap and partially abrogated by inhaled corticosteroid treatment: a bronchoscopy endobronchial biopsy study

    doi: 10.3389/fimmu.2025.1531279

    Figure Lengend Snippet: Relative expression changes of N-cadherin and E-cadherin in human-derived lung primary epithelial cells (HC and ACO patients) post induction with Th-2 cytokine IL-13, CSE and EMT activator TGF-β. # p<0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: Cigarette smoke extract (CSE) was prepared by bubbling smoke from one filter-less Kentucky research cigarette, 3R4F, which contains 9.5 mg of tar and 0.8 mg of nicotine, through 10 mL of Bronchial Epithelial Basal Medium (BEBM, Lonza) at a rate of one cigarette every 5 minutes ( ).

    Techniques: Expressing, Derivative Assay